Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design | PLOS ONE
![IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/41/Alice_Wang_Table1.png/700px-Alice_Wang_Table1.png)
IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare
![SOLVED: You will need to determine your insert amount based on the table using the NEB ligation calculator. If you do not have enough insert DNA based on the calculations, Stanley cut SOLVED: You will need to determine your insert amount based on the table using the NEB ligation calculator. If you do not have enough insert DNA based on the calculations, Stanley cut](https://cdn.numerade.com/ask_previews/4b5d69b1-7bfd-477a-8784-52a8e59aa3d5_large.jpg)
SOLVED: You will need to determine your insert amount based on the table using the NEB ligation calculator. If you do not have enough insert DNA based on the calculations, Stanley cut
![Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly | ACS Synthetic Biology Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly | ACS Synthetic Biology](https://pubs.acs.org/cms/10.1021/acssynbio.8b00333/asset/images/large/sb-2018-00333u_0007.jpeg)